Archive for February, 2008
« Previous EntriesMessenger RNA expression patterns of histone-associated genes in bovine preimplantation embryos derived from different origins.
Friday, February 29th, 2008Nowak-Imialek M, Wrenzycki C, Herrmann D, Lucas-Hahn A, Lagutina I, Lemme E, Lazzari G, Galli C, Niemann HMol Reprod Dev (May 2008)
Histone modification genes in bovine embryos: The mRNA expression pattern of histone-related genes was determined in bovine oocytes and embryos. We compared immature and in vitro-matured oocytes, either before or after enucleation and activation, in vitro produced embryos (zygotes, 8-16 cell stages, blastocysts), [...]
Developmental capacity of porcine nuclear transfer embryos correlate with levels of chromatin-remodeling transcripts in donor cells.
Friday, February 29th, 2008Magnani L, Lee K, Fodor WL, Machaty Z, Cabot RAMol Reprod Dev (May 2008)
Somatic cell nuclear transfer (SCNT) still retains important limitations. Impaired epigenetic reprogramming is considered responsible for altered gene expression and developmental failure in SCNT-derived embryos. After nuclear transfer the donor cell nucleus undergoes extensive changes in gene expression that involve epigenetic modifications and chromatin remodeling. We hypothesized [...]
Endogastric capsule for E-cadherin gene (CDH1) promoter hypermethylation assessment in DNA from gastric juice of diffuse gastric cancer patients.
Friday, February 29th, 2008Muretto P, Ruzzo A, Pizzagalli F, Graziano F, Maltese P, Zingaretti C, Berselli E, Donnarumma N, Magnani MAnn Oncol (Mar 2008)
BACKGROUND: We investigated whether an endogastric capsule (EC) may be a valuable tool for collecting DNA from exfoliated cells from the gastric mucosa and for carrying out an analysis of promoter methylation status of the E-cadherin (CDH1) gene in poorly differentiated, diffuse gastric cancer [...]
Transgenerational effects of the endocrine disruptor vinclozolin on the prostate transcriptome and adult onset disease.
Friday, February 29th, 2008Anway MD, Skinner MKProstate (Apr 2008)
PURPOSE: The ability of an endocrine disruptor exposure during gonadal sex determination to promote a transgenerational prostate disease phenotype was investigated in the current study. METHODS: Exposure of an F0 gestating female rat to the endocrine disruptor vinclozolin during F1 embryo gonadal sex determination promoted a transgenerational adult onset prostate disease phenotype. [...]
Methylation status of putative differentially methylated regions of porcine IGF2 and H19.
Friday, February 29th, 2008Han DW, Im YB, Do JT, Gupta MK, Uhm SJ, Kim JH, Schler HR, Lee HTMol Reprod Dev (May 2008)
This study was designed to identify the putative differentially methylated regions (DMRs) of the porcine imprinted genes insulin-like growth factor 2 and H19 (IGF2-H19), and to assess the genomic imprinting status of IGF2-H19 by identifying the methylation patterns of these regions in germ cells, [...]
The XVth World Congress of Psychiatric Genetics, October 7-11, 2007: Rapporteur summaries of oral presentations.
Friday, February 29th, 2008Alkelai A, Baum A, Carless M, Crowley J, Dasbanerjee T, Dempster E, Docherty S, Hare E, Galsworthy MJ, Grover D, Glubb D, Karlsson R, Mill J, Sen S, Quinones MP, Vallender EJ, Verma R, Vijayan NN, Villafuerte S, Voineskos AN, Volk H, Yu L, Zimmermann P, Delisi LEAm J Med Genet B Neuropsychiatr Genet (Mar 2008)
The World Congress of Psychiatric Genetics (WCPG) has become an annual event since the early 1990’s sponsored by the International Society of Psychiatric Genetics [...]
Cancer testis antigens in human melanoma stem cells: expression, distribution, and methylation status.
Friday, February 29th, 2008Sigalotti L, Covre A, Zabierowski S, Himes B, Colizzi F, Natali PG, Herlyn M, Maio MJ Cell Physiol (May 2008)
Neoplastic populations with stem cell potential have been most recently identified in human cutaneous melanoma, and initially characterized for their phenotypic profile. Being melanoma stem cells (MSC) the most desirable target of therapeutic intervention, we asked whether they express the epigenetically-regulated cancer testis antigens [...]
Towards an integrated understanding of the structure and mechanics of the cell nucleus.
Friday, February 29th, 2008Rowat AC, Lammerding J, Herrmann H, Aebi UBioessays (Mar 2008)
Changes in the shape and structural organization of the cell nucleus occur during many fundamental processes including development, differentiation and aging. In many of these processes, the cell responds to physical forces by altering gene expression within the nucleus. How the nucleus itself senses and responds to such mechanical cues [...]
Epigenetic regulation of Hox gene activation: the waltz of methyls.
Friday, February 29th, 2008Soshnikova N, Duboule DBioessays (Mar 2008)
Genetic studies have revealed that the antagonistic interplay between PcG and TrxG/MLL complexes is essential for the proper maintenance of vertebrate Hox gene expression in time and space. Hox genes must be silenced in totipotent embryonic stem cells and, in contrast, rapidly activated during embryogenesis. Here we discuss some recently published articles1-4 [...]
Histone deacetylase inhibitors induce TAP, LMP, Tapasin genes and MHC class I antigen presentation by melanoma cells.
Friday, February 29th, 2008Khan AN, Gregorie CJ, Tomasi TBCancer Immunol Immunother (May 2008)
Histone deacetylase inhibitors (HDACi), including trichostatin A (TSA) and valproic acid, can alter the acetylation of histones in chromatin and enhance gene transcription. Previously we demonstrated that HDACi-treated tumor cells are capable of presenting antigen via the MHC class II pathway. In this study, we show that treatment with [...]
Positive histone marks are associated with active transcription from a methylated ICSBP/IRF8 gene.
Friday, February 29th, 2008Tshuikina M, Nilsson K, Oberg FGene (Mar 2008)
Epigenetic modifications are critical for regulating many different aspects of normal cell biology and tumourigenesis. Gene expression may be epigenetically silenced by DNA-methylation and histone modifications, resulting in remodelling of chromatin into a repressed state. We performed DNA-methylation analysis of the ICSBP/IRF8 gene, a member of the IRF family of transcriptional [...]
Environment, diet and CpG island methylation: Epigenetic signals in gastrointestinal neoplasia.
Friday, February 29th, 2008Johnson IT, Belshaw NJFood Chem Toxicol (Apr 2008)
The epithelial surfaces of the mammalian alimentary tract are characterised by very high rates of cell proliferation and DNA synthesis, and in humans they are highly susceptible to cancer. The role of somatic mutations as drivers of carcinogenesis in the alimentary tract is well established, but the importance of gene [...]
Epigenetic modifications and chromatin loop organization explain the different expression profiles of the Tbrg4, WAP and Ramp3 genes.
Friday, February 29th, 2008Montazer-Torbati MB, Hue-Beauvais C, Droineau S, Ballester M, Coant N, Aujean E, Petitbarat M, Rijnkels M, Devinoy EExp Cell Res (Mar 2008)
Whey Acidic Protein (WAP) gene expression is specific to the mammary gland and regulated by lactogenic hormones to peak during lactation. It differs markedly from the more constitutive expression of the two flanking genes, Ramp3 and Tbrg4. Our results show that the tight [...]
The Hsp90 molecular chaperone: an open and shut case for treatment.
Monday, February 25th, 2008Pearl LH, Prodromou C, Workman PBiochem J (Mar 2008)
The molecular chaperone Hsp90 (90 kDa heat-shock protein) is a remarkably versatile protein involved in the stress response and in normal homoeostatic control mechanisms. It interacts with ‘client proteins’, including protein kinases, transcription factors and others, and either facilitates their stabilization and activation or directs them for proteasomal degradation. By [...]
The power of the word may reside in the power of affect.
Monday, February 25th, 2008Panksepp JIntegr Psychol Behav Sci (Mar 2008)
This commentary on Dan Shanahan’s, A New View of Language, Emotion and the Brain, basically agrees with an emotion-based view of the evolutionary and developmental basis of language acquisition. It provides a supplementary neuroscience perspective that is more deeply affective and epigenetic in the sense that all claims about neocortically-based [...]
