research articles
« Previous EntriesPotentiation of reactive oxygen species is a marker for synergistic cytotoxicity of MS-275 and 5-azacytidine in leukemic cells.
Sunday, March 30th, 2008Gao S, Mobley A, Miller C, Boklan J, Chandra JLeuk Res (May 2008)
Epigenetic modifiers are currently in clinical use for various tumor types. Recently, numerous studies supporting the combination of histone deacetylase inhibitors (HDACi) and DNA methyltransferase inhibitors have emerged, encouraging early clinical trials of these agents together. Here we show that MS-275, an HDACi, and 5-azacytidine, a methyltransferase inhibitor, [...]
Proliferative inflammatory atrophy: a background lesion of prostate cancer?
Sunday, March 30th, 2008Woenckhaus J, Fenic IAndrologia (Apr 2008)
Proliferative inflammatory atrophy (PIA) belongs to the atrophic lesions that frequently occur in the prostate. The location of PIA in the periphery of the gland near to prostate carcinoma or even showing direct transition to malignant or pre-malignant epithelia suggested a connection between PIA and prostate cancer. Further findings in PIA, such [...]
A quantitative high-throughput screen identifies potential epigenetic modulators of gene expression.
Sunday, March 30th, 2008Johnson RL, Huang W, Jadhav A, Austin CP, Inglese J, Martinez EDAnal Biochem (Apr 2008)
Epigenetic regulation of gene expression is essential in embryonic development and contributes to cancer pathology. We used a cell-based imaging assay that measures derepression of a silenced green fluorescent protein (GFP) reporter to identify novel classes of compounds involved in epigenetic regulation. This locus derepression (LDR) assay [...]
Molecular and cytogenetic evidence for an allotetraploid origin of Trifolium dubium (Leguminosae).
Sunday, March 30th, 2008Ansari HA, Ellison NW, Williams WMChromosoma (Apr 2008)
Suckling clover, Trifolium dubium Sibth., is a European grassland legume that has spread to many parts of the world. The present work shows that it is an allotetraploid (2n = 4x = 30) combining the genomes of T. campestre Schreb. (2n = 2x = 14) and T. micranthum Viv. (2n = [...]
The current state of preclinical prostate cancer animal models.
Sunday, March 30th, 2008Pienta KJ, Abate-Shen C, Agus DB, Attar RM, Chung LW, Greenberg NM, Hahn WC, Isaacs JT, Navone NM, Peehl DM, Simons JW, Solit DB, Soule HR, Vandyke TA, Weber MJ, Wu L, Vessella RLProstate (May 2008)
Prostate cancer continues to be a major cause of morbidity and mortality in men around the world. The field of prostate cancer research continues to be hindered by the lack of relevant preclinical models to study tumorigenesis [...]
Epigenetic-based treatments emphasize the biologic differences of core-binding factor acute myeloid leukemias.
Sunday, March 30th, 2008Serrano E, Carnicer MJ, Lasa A, Orantes V, Pena J, Brunet S, Aventn A, Sierra J, Nomdedu JFLeuk Res (Jun 2008)
Acute myeloid leukemia (AML) is a heterogeneous group of disorders characterized by an abnormal proliferation of the myeloid precursors and a maturation block. The most common chromosomal lesions in AML are the t(8;21) and inv(16). To better understand the leukemogenic mechanism of these fusion [...]
Promoter methylation correlates with reduced Smad4 expression in advanced prostate cancer.
Sunday, March 30th, 2008Aitchison AA, Veerakumarasivam A, Vias M, Kumar R, Hamdy FC, Neal DE, Mills IGProstate (May 2008)
BACKGROUND: Transforming growth factor-beta (TGF-beta) is a potent growth inhibitor in a wide range of cell types. A transducer of TGF-beta signaling known as Mothers against decapentaplegic homologue 4 (Smad4) is a known tumor suppressor found on chromosome 18q21.1 and is typically inactivated by deletion or mutation [...]
Helicobacter pylori regulates p21(WAF1) by histone H4 acetylation.
Sunday, March 30th, 2008Xia G, Schneider-Stock R, Diestel A, Habold C, Krueger S, Roessner A, Naumann M, Lendeckel UBiochem Biophys Res Commun (May 2008)
Helicobacter pylori are bacteria that colonize the stomach persistently, which confers risk of serious diseases, including peptic ulceration and gastric neoplasia. Aberrant expression of cell cycle control proteins has been demonstrated in H. pylori infected gastric epithelial cells, suggesting that perturbation of the [...]
Review. Meiotic drive and sex determination: molecular and cytological mechanisms of sex ratio adjustment in birds.
Sunday, March 30th, 2008Rutkowska J, Badyaev AVPhilos Trans R Soc Lond B Biol Sci (May 2008)
Differences in relative fitness of male and female offspring across ecological and social environments should favour the evolution of sex-determining mechanisms that enable adjustment of brood sex ratio to the context of breeding. Despite the expectation that genetic sex determination should not produce consistent bias [...]
The effects of trichostatin a on mRNA expression of chromatin structure-, DNA methylation-, and development-related genes in cloned mouse blastocysts.
Sunday, March 30th, 2008Li X, Kato Y, Tsuji Y, Tsunoda YCloning Stem Cells (2008)
Trichostatin A (TSA) is the most potent histone deacetylase (HDAC) inhibitor known. We previously reported that treatment of mouse somatic cell nuclear-transferred (SCNT) oocytes with TSA significantly increased the blastocyst rate, blastocyst cell number, and full-term development. How TSA enhances the epigenetic remodeling ability of somatic nuclei and the [...]
Enhanced Plating Efficiency of Trypsin-Adapted Human Embryonic Stem Cells is Reversible and Independent of Trisomy 12/17.
Sunday, March 30th, 2008Chan EM, Yates F, Boyer LF, Schlaeger TM, Daley GQCloning Stem Cells (2008)
Human embryonic stem cells (hESCs) can be cultured abundantly and indefinitely, but are subject to accumulations of chromosomal aberrations. To preserve their genetic integrity, hESCs are commonly maintained as cell aggregates or clumps during passaging. However, clump passaging hinders large-scale culture and complicates the isolation of single cell [...]
NGF/PI3K signaling-mediated epigenetic regulation of delta opioid receptor gene expression.
Sunday, March 30th, 2008Chen YL, Law PY, Loh HHBiochem Biophys Res Commun (Apr 2008)
The G protein-coupled delta opioid receptor gene (dor) has been associated with neuronal survival, differentiation, and neuroprotection. Our previous study identified PI3K/Akt/NF-kappaB signaling is a main downstream signaling pathway in nerve growth factor (NGF)-induced temporal expression of the dor gene in the PC12 cell model. It is still [...]
Genetic evidence that Ras-like GTPases, Gtr1p, and Gtr2p, are involved in epigenetic control of gene expression in Saccharomyces cerevisiae.
Sunday, March 30th, 2008Sekiguchi T, Hayashi N, Wang Y, Kobayashi HBiochem Biophys Res Commun (Apr 2008)
Gtr1p and Gtr2p of Saccharomyces cerevisiae are members of the Ras-like GTP binding family and interact genetically with Prp20p (yeast RCC1), which is a guanine nucleotide exchange factor for Gsp1p (yeast homolog of Ran, involved in nuclear export). Recently, Gtr1p and Gtr2p were suggested to be molecular [...]
Promoter analysis of epigenetically controlled genes in bladder cancer.
Sunday, March 30th, 2008Veerla S, Panagopoulos I, Jin Y, Lindgren D, Hglund MGenes Chromosomes Cancer (May 2008)
DNA methylation is an important epigenetic modification that regulates several genes crucial for tumor development. To identify epigenetically regulated genes in bladder cancer, we performed genome wide expression analyses of eight-bladder cancer cell lines treated with the demethylating agents 5-aza-2′-cytidine and zebularine. To identify methylated C-residues, we [...]
Developmental capacity of porcine nuclear transfer embryos correlate with levels of chromatin-remodeling transcripts in donor cells.
Monday, March 3rd, 2008Magnani L, Lee K, Fodor WL, Machaty Z, Cabot RAMol Reprod Dev (May 2008)
Somatic cell nuclear transfer (SCNT) still retains important limitations. Impaired epigenetic reprogramming is considered responsible for altered gene expression and developmental failure in SCNT-derived embryos. After nuclear transfer the donor cell nucleus undergoes extensive changes in gene expression that involve epigenetic modifications and chromatin remodeling. We hypothesized [...]
